primer88Primer3 was a complete re-implementation of an earlier program: Primer 0.5 (Steve Lincoln, Mark Daly, and Eric S. Lander). Lincoln Stein championed the idea of making Primer3 a softwareDesn PCR and qPCR assays intellently for any species, with PrimerQuest. Fine-tune primer and probe desn by adjusting the tool’s custom desn parameters.